Rational design of nonnatural peptides as high-affinity ligands for the HLA-B*2705 human leukocyte antigen.
Article Details
- CitationCopy to clipboard
Rognan D, Scapozza L, Folkers G, Daser A
Rational design of nonnatural peptides as high-affinity ligands for the HLA-B*2705 human leukocyte antigen.
Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):753-7.
- PubMed ID
- 7846047 [ View in PubMed]
- Abstract
From the three-dimensional structure of the class I major histocompatibility complex (MHC) HLA-B*2705 protein, several nonnatural peptides were designed either to optimize the interactions of one peptide amino acid (position 3) with its HLA binding pocket (pocket D) or to simplify the T-cell receptor-binding part by substitution with organic spacers. The stability of each MHC-ligand complex was simulated by 150-ps molecular dynamics in a water environment and compared with that of the natural complexes. All peptides were synthesized and tested for binding to the class I MHC protein in an in vitro assembly assay. As predicted from the computed atomic fluctuations and buried surface areas of MHC-bound ligands, bulky hydrophobic side chains at position 3 enhance the binding of a nonameric peptide to the HLA-B27 protein. Furthermore, it was possible to simplify half of the peptide sequence (residues 4-8) by replacement with organic fragments without altering the affinity of the designed ligands for the class I MHC protein. This study constitutes an initial step toward the rational design of nonpeptide class I MHC ligands for use in the selective immunotherapy of autoimmune diseases associated with particular HLA alleles.