Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes.
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Cantalupo G, Alifano P, Roberti V, Bruni CB, Bucci C
Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes.
EMBO J. 2001 Feb 15;20(4):683-93.
- PubMed ID
- 11179213 [ View in PubMed]
- Abstract
Rab7 is a small GTPase that controls transport to endocytic degradative compartments. Here we report the identification of a novel 45 kDa protein that specifically binds Rab7GTP at its C-terminus. This protein contains a domain comprising two coiled-coil regions typical of myosin-like proteins and is found mainly in the cytosol. We named it RILP (Rab-interacting lysosomal protein) since it can be recruited efficiently on late endosomal and lysosomal membranes by Rab7GTP. RILP-C33 (a truncated form of the protein lacking the N-terminal half) strongly inhibits epidermal growth factor and low-density lipoprotein degradation, and causes dispersion of lysosomes similarly to Rab7 dominant-negative mutants. More importantly, expression of RILP reverses/prevents the effects of Rab7 dominant-negative mutants. All these data are consistent with a model in which RILP represents a downstream effector for Rab7 and both proteins act together in the regulation of late endocytic traffic.