Cloning and expression of a human voltage-gated potassium channel. A novel member of the RCK potassium channel family.
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Grupe A, Schroter KH, Ruppersberg JP, Stocker M, Drewes T, Beckh S, Pongs O
Cloning and expression of a human voltage-gated potassium channel. A novel member of the RCK potassium channel family.
EMBO J. 1990 Jun;9(6):1749-56.
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- 2347305 [ View in PubMed]
- Abstract
We have isolated and characterized a human cDNA (HBK2) that is homologous to novel member (RCK2) of the K+ channel RCK gene family expressed in rat brain. RCK2 mRNA was detected predominantly in midbrain areas and brainstem. The primary sequences of the HBK2/RCK2 K+ channel proteins exhibit major differences to other members of the RCK gene family. The bend region between segments S1 and S2 is unusually long and does not contain the N-glycosylation site commonly found in this region. They might be O-glycosylated instead. Functional characterization of the HBK2/RCK2 K+ channels in Xenopus laevis oocytes following micro-injection in in vitro transcribed HBK2 or RCK2 cRNA showed that the HBK2/RCK2 proteins form voltage-gated K+ channels with novel functional and pharmacological properties. These channels are different to RCK1, RCK3, RCK4 and RCK5 K+ channels.