Structural insights into the autoactivation mechanism of p21-activated protein kinase.
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Wang J, Wu JW, Wang ZX
Structural insights into the autoactivation mechanism of p21-activated protein kinase.
Structure. 2011 Dec 7;19(12):1752-61. doi: 10.1016/j.str.2011.10.013.
- PubMed ID
- 22153498 [ View in PubMed]
- Abstract
p21-activated kinases (PAKs) play an important role in diverse cellular processes. Full activation of PAKs requires autophosphorylation of a critical threonine/serine located in the activation loop of the kinase domain. Here we report crystal structures of the phosphorylated and unphosphorylated PAK1 kinase domain. The phosphorylated PAK1 kinase domain has a conformation typical of all active protein kinases. Interestingly, the structure of the unphosphorylated PAK1 kinase domain reveals an unusual dimeric arrangement expected in an authentic enzyme-substrate complex, in which the activation loop of the putative "substrate" is projected into the active site of the "enzyme." The enzyme is bound to AMP-PNP and has an active conformation, whereas the substrate is empty and adopts an inactive conformation. Thus, the structure of the asymmetric homodimer mimics a trans-autophosphorylation complex, and suggests that unphosphorylated PAK1 could dynamically adopt both the active and inactive conformations in solution.