Application of two-phase hollow fiber liquid phase microextraction coupled with high-performance liquid chromatography for the study of the echinacoside pharmacokinetics in Parkinson's disease rat plasma.

Article Details

Citation Copy to clipboard

Zhou J, Zeng P, Sun JB, Wang FQ, Zhang Q

J Pharm Biomed Anal. 2013 Jul-Aug;81-82:27-33. doi: 10.1016/j.jpba.2013.03.020. Epub 2013 Apr 3.

PubMed ID

23591053 [ View in PubMed

]

Abstract

A simple and solvent-minimized sample preparation technique based on two-phase hollow fiber liquid phase microextraction (HF-LPME) was developed and used to quantify the echinacoside in Parkinson's disease rat plasma following oral administration. Analysis was accomplished by reversed-phase high performance liquid chromatography (HPLC) with ultraviolet detection. The influence factors relevant to the HF-LPME processes were optimized. Under the optimized conditions, the preconcentration factor for echinacoside was 337. Calibration curves with reasonable linearity (r(2) >/= 0.9998) were obtained in the range of 5-750 ng mL(-1). Intra-day and inter-day precision (RSD) were

DrugBank Data that Cites this Article

Drugs: Echinacoside