A role for divalent metal transporter (DMT1) in mitochondrial uptake of iron and manganese.

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Wolff NA, Garrick MD, Zhao L, Garrick LM, Ghio AJ, Thevenod F

A role for divalent metal transporter (DMT1) in mitochondrial uptake of iron and manganese.

Sci Rep. 2018 Jan 9;8(1):211. doi: 10.1038/s41598-017-18584-4.

PubMed ID

29317744 [ View in PubMed

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Abstract

Much of iron and manganese metabolism occurs in mitochondria. Uptake of redox-active iron must be tightly controlled, but little is known about how metal ions enter mitochondria. Recently, we established that the divalent metal transporter 1 (DMT1) is present in the outer mitochondrial membrane (OMM). Therefore we asked if it mediates Fe(2+) and Mn(2+) influx. Mitochondria were isolated from HEK293 cells permanently transfected with inducible rat DMT1 isoform 1 A/+IRE (HEK293-rDMT1). Fe(2+)-induced quenching of the dye PhenGreenSK (PGSK) occurred in two phases, one of which reflected OMM DMT1 with stronger Fe(2+) uptake after DMT1 overexpression. DMT1-specific quenching showed an apparent affinity of ~1.5 microM for Fe(2+)and was blocked by the DMT1 inhibitor CISMBI. Fe(2+) influx reflected an imposed proton gradient, a response that was also observed in purified rat kidney cortex (rKC) mitochondria. Non-heme Fe accumulation assayed by ICPOES and stable (57)Fe isotope incorporation by ICPMS were increased in HEK293-rDMT1 mitochondria. HEK293-rDMT1 mitochondria displayed higher (59)Fe(2+) and (54)Mn(2+) uptake relative to controls with (54)Mn(2+) uptake blocked by the DMT1 inhibitor XEN602. Such transport was defective in rKC mitochondria with the Belgrade (G185R) mutation. Thus, these results support a role for DMT1 in mitochondrial Fe(2+) and Mn(2+) acquisition.

DrugBank Data that Cites this Article

Drug Transporters

Drug	Transporter	Kind	Organism	Pharmacological Action	Actions
Ferrous sulfate anhydrous	Natural resistance-associated macrophage protein 2	Protein	Humans	Unknown	Substrate	Details