Anvirzel(TM)regulates cell death through inhibiting GSK-3 activity in human U87 glioma cells.

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Terzioglu-Usak S, Nalli A, Elibol B, Ozek E, Hatiboglu MA

Anvirzel(TM)regulates cell death through inhibiting GSK-3 activity in human U87 glioma cells.

Neurol Res. 2020 Jan;42(1):68-75. doi: 10.1080/01616412.2019.1709744. Epub 2020 Jan 3.

PubMed ID

31900072 [ View in PubMed

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Abstract

Objectives: Cardiac glycosides are used as potential anti-cancer agents due to their effects on the inhibition of proliferation and induction of apoptosis and/or autophagy in cancer cells. Herein, we aimed to study the potential signaling pathways taken role in differential cell-death properties of Anvirzel(TM) which is consisted of two toxic cardiac glycosides (oleandrin and oleandrigenin), in U87 human glioblastoma cells.Methods: The anti-proliferative and anti-migratory effects of Anvirzel(TM) were assessed in U87 cells by WST-1 assay and wound healing assay, respectively. After treatment of Anvirzel(TM)with doses of 10, 25, 50, 100 and 250 mug/ml, expression levels of proteins related to cell death were investigated by Western blot.Results: Anvirzel markedly inhibited the growth of U87 cells in a time- and dose-dependent manner following 24 h and 48 h treatments (p < 0.05). In addition, it was found that Anvirzel inhibited GSK-3, NOS and HIF1-alpha expressions whereas activated ERK in U87 cells compared to vehicle (p < 0.05).Discussion: The results suggested that Anvirzel(TM) regulated cell death distinctly from apoptosis in human glioblastoma cells. Further studies are required for validation of mechanistic insights about the potential signaling pathways taken role in differential cell death properties of Anvirzel(TM).

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Oleandrin	Ephrin type-B receptor 2	Protein	Humans	Unknown	Activator	Details