A single step purification for autolytic zinc proteinases.

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Wilfong EM, Locklear U, Toone EJ

A single step purification for autolytic zinc proteinases.

Bioorg Med Chem Lett. 2010 Jan 1;20(1):280-2. doi: 10.1016/j.bmcl.2009.10.114. Epub 2009 Nov 5.

PubMed ID

19942433 [ View in PubMed

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Abstract

We describe a novel single-step method for the purification of stromelysin-1 catalytic domain (SCD) via immobilized metal affinity chromatography under denaturing conditions that inhibit proteolytic activity followed by on-column refolding and spontaneous autolysis of the fusion peptide to yield pure, active stromelysin-1 catalytic domain. The methodology provides a general approach for the rapid purification of large quantities of zinc proteinases.

DrugBank Data that Cites this Article

Binding Properties

Drug	Target	Property	Measurement	pH	Temperature (°C)
3-[(4'-cyanobiphenyl-4-yl)oxy]-N-hydroxypropanamide	Stromelysin-1	Kd (nM)	100	N/A	N/A	Details
3-[(4'-cyanobiphenyl-4-yl)oxy]-N-hydroxypropanamide	Stromelysin-1	Kd (nM)	110	N/A	N/A	Details