Chronic or delayed treatment with an oral dithiocarbamate analog decreases glycation and protects diabetic arteries.
Article Details
- CitationCopy to clipboard
Pieper GM, Siebeneich W, Olds CL, Lai CS
Chronic or delayed treatment with an oral dithiocarbamate analog decreases glycation and protects diabetic arteries.
Eur J Pharmacol. 2003 Jul 4;472(1-2):127-34.
- PubMed ID
- 12860481 [ View in PubMed]
- Abstract
In the present study, we examined the efficacy of a dithiocarbamate-based compound, denoted as NOX-700, on diabetes-induced endothelial dysfunction and glycosylation of hemoglobin (Hb). Streptozotocin-induced diabetic rats received 3 mg/ml NOX-700 in drinking water beginning at 72 h or 4 weeks and continued to 8 weeks. Oxidative and glycooxidative stress were examined by electrophoretic mobility shift assay (EMSA) for nuclear factor-kappaB (NF-kappaB) in nuclear fractions of aortic homogenates and by glycosylated Hb, respectively. Vascular reactivity was examined in aortic ring segments ex vivo. Treatment with NOX-700 inhibited glycosylated Hb formation when given long-term or after delayed administration. NOX-700 improved endothelium-dependent relaxation to acetylcholine but did not alter reactivity to norepinephrine or nitroglycerin, suggesting selective protection of the endothelium. Nuclear factor-kappaB (NF-kappaB) nuclear binding activity was significantly increased in diabetic aortas and abrogated by NOX-700. Thus, vascular protection by NOX-700 is believed to be mediated, in part, by an antioxidant mechanism and decreased protein glycation.
DrugBank Data that Cites this Article
- Drugs