Mapping of the second tetracycline binding site on the ribosomal small subunit of E.coli.
Article Details
- CitationCopy to clipboard
Anokhina MM, Barta A, Nierhaus KH, Spiridonova VA, Kopylov AM
Mapping of the second tetracycline binding site on the ribosomal small subunit of E.coli.
Nucleic Acids Res. 2004 May 11;32(8):2594-7. doi: 10.1093/nar/gkh583. Print 2004.
- PubMed ID
- 15141029 [ View in PubMed]
- Abstract
Tetracycline blocks stable binding of aminoacyl-tRNA to the bacterial ribosomal A-site. Various tetracycline binding sites have been identified in crystals of the 30S ribosomal small subunit of Thermus thermophilus. Here we describe a direct photo- affinity modification of the ribosomal small subunits of Escherichia coli with 7-[3H]-tetracycline. To select for specific interactions, an excess of the 30S subunits over tetracycline has been used. Primer extension analysis of the 16S rRNA revealed two sites of the modifications: C936 and C948. Considering available data on tetracycline interactions with the prokaryotic 30S subunits, including the presented data (E.coli), X-ray data (T.thermophilus) and genetic data (Helicobacter pylori, E.coli), a second high affinity tetracycline binding site is proposed within the 3'-major domain of the 16S rRNA, in addition to the A-site related tetracycline binding site.
DrugBank Data that Cites this Article
- Drug Targets
Drug Target Kind Organism Pharmacological Action Actions Omadacycline 16S ribosomal RNA Nucleotide Enteric bacteria and other eubacteria YesInhibitorDetails Omadacycline 30S ribosomal protein S14 Protein Escherichia coli (strain K12) YesInhibitorDetails Omadacycline 30S ribosomal protein S19 Protein Escherichia coli (strain K12) YesInhibitorDetails Omadacycline 30S ribosomal protein S3 Protein Escherichia coli (strain K12) YesInhibitorDetails Omadacycline 30S ribosomal protein S7 Protein Escherichia coli (strain K12) YesInhibitorDetails Omadacycline 30S ribosomal protein S8 Protein Escherichia coli (strain K12) YesInhibitorDetails