Isolation and sequence determination of cDNA encoding human T-protein of the glycine cleavage system.
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Hayasaka K, Nanao K, Takada G, Okamura-Ikeda K, Motokawa Y
Isolation and sequence determination of cDNA encoding human T-protein of the glycine cleavage system.
Biochem Biophys Res Commun. 1993 Apr 30;192(2):766-71.
- PubMed ID
- 7916605 [ View in PubMed]
- Abstract
We present the full-length cDNA sequence of human T-protein of the glycine cleavage system. Overlapping cDNA clones were isolated by screening human liver cDNA libraries with bovine T-protein cDNA. The 1209 base pair open reading frame encodes 403 amino acid precursor protein, and the deduced amino acid sequence of the mature peptide shows 90 and 68% homology to that of bovine and chicken counterpart, respectively. RNA blot analysis of human liver transcripts and Southern blot analysis of human genomic DNA confirm that human T-protein is encoded by a single gene.