Quantitative detection of single amino acid polymorphisms by targeted proteomics.
Article Details
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Su ZD, Sun L, Yu DX, Li RX, Li HX, Yu ZJ, Sheng QH, Lin X, Zeng R, Wu JR
Quantitative detection of single amino acid polymorphisms by targeted proteomics.
J Mol Cell Biol. 2011 Oct;3(5):309-15. doi: 10.1093/jmcb/mjr024.
- PubMed ID
- 22028381 [ View in PubMed]
- Abstract
Single-nucleotide polymorphisms (SNPs) are recognized as one kind of major genetic variants in population scale. However, polymorphisms at the proteome level in population scale remain elusive. In the present study, we named amino acid variances derived from SNPs within coding regions as single amino acid polymorphisms (SAPs) at the proteome level, and developed a pipeline of non-targeted and targeted proteomics to identify and quantify SAP peptides in human plasma. The absolute concentrations of three selected SAP-peptide pairs among 290 Asian individuals were measured by selected reaction monitoring (SRM) approach, and their associations with both obesity and diabetes were further analyzed. This work revealed that heterozygotes and homozygotes with various SAPs in a population could have different associations with particular traits. In addition, the SRM approach allows us for the first time to separately measure the absolute concentration of each SAP peptide in the heterozygotes, which also shows different associations with particular traits.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID Prothrombin P00734 Details Complement C5 P01031 Details Apolipoprotein E P02649 Details Complement C1r subcomponent P00736 Details Apolipoprotein A-I P02647 Details Complement C3 P01024 Details Complement factor H P08603 Details Pigment epithelium-derived factor P36955 Details Apolipoprotein B-100 P04114 Details