Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase.
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Jolly DJ, Okayama H, Berg P, Esty AC, Filpula D, Bohlen P, Johnson GG, Shively JE, Hunkapillar T, Friedmann T
Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase.
Proc Natl Acad Sci U S A. 1983 Jan;80(2):477-81.
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- 6300847 [ View in PubMed]
- Abstract
We have cloned a full-length 1.6-kilobase cDNA of a human mRNA coding for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) into a simian virus 40-based expression vector and have determined its full nucleotide sequence. The inferred amino acid sequence agrees with a partial amino acid sequence determined for authentic human HPRT protein. Transfection of HPRT-deficient mouse LA9 cells with the purified plasmid leads to the expression of human HPRT enzyme activity in cells stably transfected and selected for enzyme activity in hypoxanthine/aminopterin/thymidine medium.