Identification of negative and positive regulatory elements in the human renin gene.
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Burt DW, Nakamura N, Kelley P, Dzau VJ
Identification of negative and positive regulatory elements in the human renin gene.
J Biol Chem. 1989 May 5;264(13):7357-62.
- PubMed ID
- 2540188 [ View in PubMed]
- Abstract
Renin gene expression is tissue-specific and under complex hormonal control. To investigate which DNA elements are involved in the control of human renin gene expression, we performed transient DNA transfer experiments with renin-chloramphenicol acetyltransferase fusions. We have mapped a complex arrangement of positive and negative control sequences in the 5' flanking region of the human renin gene. One positive control element is active in either orientation and defines a renin gene enhancer. The negative element is also active in either orientation and defines a renin gene silencer. Mapping in the same region as the silencer is a cAMP-responsive element, a sequence conserved in mouse, rat, and human renin genes.