Integral and associated lysosomal membrane proteins.

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Schroder B, Wrocklage C, Pan C, Jager R, Kosters B, Schafer H, Elsasser HP, Mann M, Hasilik A

Integral and associated lysosomal membrane proteins.

Traffic. 2007 Dec;8(12):1676-86. Epub 2007 Sep 26.

PubMed ID
17897319 [ View in PubMed
]
Abstract

We searched for novel proteins in lysosomal membranes, tentatively participating in molecular transport across the membrane and/or in interactions with other compartments. In membranes purified from placental lysosomes, we identified 58 proteins, known to reside at least partially in the lysosomal membrane. These included 17 polypeptides comprising or associated with the vacuolar adenosine triphosphatase. We report on additional 86 proteins that were significantly enriched in the lysosomal membrane fraction. Among these, 12 novel proteins of unknown functions were found. Three were orthologues of rat proteins that have been identified in tritosomes by Bagshaw RD et al. (A proteomic analysis of lysosomal integral membrane proteins reveals the diverse composition of the organelle. Mol Cell Proteomics 2005;4:133-143). Here, the proteins encoded by LOC201931 (FLJ38482) and LOC51622 (C7orf28A) were expressed with an appended fluorescent tag in HeLa cells and found to be present in lysosomal organelles. Among the lysosomally enriched proteins, also 16 enzymes and transporters were detected that had not been assigned to lysosomal membranes previously. Finally, our results identified a particular set of proteins with known functions in signaling and targeting to be at least partially associated with lysosomes.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
CystinosinO60931Details
Proton-coupled amino acid transporter 1Q7Z2H8Details
Lysosomal alpha-glucosidaseP10253Details
GlucosylceramidaseP04062Details
Prostatic acid phosphataseP15309Details
Zinc transporter 2Q9BRI3Details
Sphingosine kinase 2Q9NRA0Details
Equilibrative nucleoside transporter 3Q9BZD2Details