Active site of human liver aldehyde dehydrogenase.
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Abriola DP, Fields R, Stein S, MacKerell AD Jr, Pietruszko R
Active site of human liver aldehyde dehydrogenase.
Biochemistry. 1987 Sep 8;26(18):5679-84.
- PubMed ID
- 3676276 [ View in PubMed]
- Abstract
Bromoacetophenone (2-bromo-1-phenylethanone) functions as an affinity reagent for human aldehyde dehydrogenase (EC 1.2.1.3) and has been found specifically to label a unique tryptic peptide in the enzyme. Amino-terminal sequence analysis of the labeled peptide after purification by two different procedures revealed the following sequence: Val-Thr-Leu-Glu-Leu-Gly-Gly-Lys. Radioactivity was found to be associated with the glutamate residue, which was identified as Glu-268 by reference to the known amino acid sequence. This paper constitutes the first identification of an active site of aldehyde dehydrogenase.