Isolation and characterization of human platelet glycoprotein IX.
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Roth GJ, Ozols J, Nugent DJ, Williams SA
Isolation and characterization of human platelet glycoprotein IX.
Biochem Biophys Res Commun. 1988 Oct 31;156(2):931-9.
- PubMed ID
- 3056407 [ View in PubMed]
- Abstract
Human platelet glycoprotein IX is a small (Mr 17,000) surface glycoprotein present in the plasma membrane as a 1:1 non-covalent complex with glycoprotein Ib (Mr 165,000). Glycoprotein IX was purified to near homogeneity by sequential wheat germ agglutinin and immuno-affinity chromatography, followed by gel filtration chromatography under denaturing conditions. Purified glycoprotein IX was characterized by determination of its amino acid composition and its NH2-terminal amino acid sequence (thr-lys-asp-xxx-pro-ser-xxx-leu-thr-(thr)2-arg-ala-(leu)-(glu)-xxx-met- gly), and monospecific anti-glycoprotein IX antibody was prepared. The study outlines a useful approach for separating and characterizing glycoprotein IX, free from other members of the glycoprotein Ib complex.