Synthesis of human fibroblast interferon by E. coli.
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Goeddel DV, Shepard HM, Yelverton E, Leung D, Crea R, Sloma A, Pestka S
Synthesis of human fibroblast interferon by E. coli.
Nucleic Acids Res. 1980 Sep 25;8(18):4057-74.
- PubMed ID
- 6159584 [ View in PubMed]
- Abstract
A cDNA library was constructed using mRNA from human fibroblasts induced with poly(I):poly(C). A bacterial clone containing fibroblast interferon cDNA sequences was identified by hybridization to a cDNA probe synthesized using deoxyoligonucleotide primers which hybridize to fibroblast interferon mRNA specifically. Expression plasmids were constructed which permitted the synthesis in E. coli of 8 x 10(7) units of human fibroblast interferon per liter of culture. The bacterially produced fibroblast interferon is indistinguishable from authentic human fibroblast interferon by several criteria.