[cDNA cloning and sequence analysis of human lactoferrin].
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Cheng H, Chen XZ, Huan LD
[cDNA cloning and sequence analysis of human lactoferrin].
Sheng Wu Gong Cheng Xue Bao. 2001 Jul;17(4):385-7.
- PubMed ID
- 11702692 [ View in PubMed]
- Abstract
Human lactoferrin (hLF) cDNA was amplified by RT-PCR from normal human mammary tissue obtained from Daxing County of Beijing City of China, and then subcloned into pGEM-T vector. hLF cDNA sequence was determined, which consists of 2136 bp. Comparison with five other hLF cDNA sequences registered in GenBank shows 99% homology in DNA sequence. However, there are two base substitutions (nucleotide 1740 G-->C, nucleotide 1756 T-->C), one of which subsequently leads to an amino acid change (residue 580 Glu-->Asp).