Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase.
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Bolduc JM, Dyer DH, Scott WG, Singer P, Sweet RM, Koshland DE Jr, Stoddard BL
Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase.
Science. 1995 Jun 2;268(5215):1312-8.
- PubMed ID
- 7761851 [ View in PubMed]
- Abstract
Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution were used to solve the structures of two sequential intermediates formed during the catalytic actions of isocitrate dehydrogenase. Both intermediates are distinct from the enzyme-substrate and enzyme-product complexes. Mutation of key catalytic residues changed the rate determining steps so that protein and substrate intermediates within the overall reaction pathway could be visualized.