A gene encoding the 16-kDa proteolipid subunit of Enterococcus hirae Na(+)-ATPase complex.
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Kakinuma Y, Kakinuma S, Takase K, Konishi K, Igarashi K, Yamato I
A gene encoding the 16-kDa proteolipid subunit of Enterococcus hirae Na(+)-ATPase complex.
Biochem Biophys Res Commun. 1993 Sep 15;195(2):1063-9.
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- 8373385 [ View in PubMed]
- Abstract
By further sequencing the cloned genome DNA of Enterococcus hirae that retains the genes encoding two major subunits of Na(+)-transport ATPase (Takase, K., Yamato, I., and Kakinuma, Y. (1993) J. Biol. Chem. 268, 11610-11616), we found a gene, ntpK, encoding a very hydrophobic protein of 156 amino acids (Mr = 16,036). The amino acid sequence deduced from the ntpK gene matched with the partial sequence of a 16-kDa protein purified as the proteolipid component of Na(+)-ATPase. The amino acid sequence and the hydropathy profile of the NtpK product were very similar with those of the 16-kDa proteolipids of vacuolar (V-) H(+)-ATPases in eukaryotes. The amino-terminal half of this sequence was highly homologous to the carboxyl-terminal half, suggesting that it was evolved from a common ancestral gene through duplication. The proteolipid of E. hirae Na(+)-ATPase belongs to that of the eukaryotic V-ATPase.