Molecular cloning of the human gene encoding lanosterol synthase from a liver cDNA library.
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Baker CH, Matsuda SP, Liu DR, Corey EJ
Molecular cloning of the human gene encoding lanosterol synthase from a liver cDNA library.
Biochem Biophys Res Commun. 1995 Aug 4;213(1):154-60.
- PubMed ID
- 7639730 [ View in PubMed]
- Abstract
Lanosterol synthase [(S)-2,3-epoxysqualene mutase (cyclizing, lanosterol forming), EC 5.4.99.7] catalyzes the cyclization of (S)-2,3-oxidosqualene to lanosterol in the reaction that forms the sterol nucleus. We report herein the cloning and characterization of the human gene (OSC) encoding lanosterol synthase, a predicted 83 kDa protein of 732 amino acids. The deduced amino acid sequence is 36-40% identical to known yeast and plant homologues and 83% identical to Rattus norvegicus lanosterol synthase. The new gene was shown to encode lanosterol synthase. The yeast lanosterol synthase deficient mutant SMY8 was complemented by the human gene, and a cell-free homogenate of SMY8 expressing the human gene was shown to convert 2,3-oxidosqualene to lanosterol.