Structures of apo and complexed Escherichia coli glycinamide ribonucleotide transformylase.
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Almassy RJ, Janson CA, Kan CC, Hostomska Z
Structures of apo and complexed Escherichia coli glycinamide ribonucleotide transformylase.
Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6114-8.
- PubMed ID
- 1631098 [ View in PubMed]
- Abstract
The three-dimensional structure of phosphoribosylglycinamide formyltransferase (10-formyltetrahydrofolate:5'-phosphoribosylglycinamide formyltransferase, EC 2.1.2.2) has been solved both as an apoenzyme at 2.8-A resolution and as a ternary complex with the substrate glycinamide ribonucleotide and a folate inhibitor at 2.5-A resolution. The structure is a modified doubly wound alpha/beta sheet with flexibility in the active site, including a disordered loop in the apo structure, which is ordered in the ternary complex structure. This enzyme is a target for anti-cancer therapy and now for structure-based drug design.