DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae.
Article Details
- CitationCopy to clipboard
Heidelberg JF, Eisen JA, Nelson WC, Clayton RA, Gwinn ML, Dodson RJ, Haft DH, Hickey EK, Peterson JD, Umayam L, Gill SR, Nelson KE, Read TD, Tettelin H, Richardson D, Ermolaeva MD, Vamathevan J, Bass S, Qin H, Dragoi I, Sellers P, McDonald L, Utterback T, Fleishmann RD, Nierman WC, White O, Salzberg SL, Smith HO, Colwell RR, Mekalanos JJ, Venter JC, Fraser CM
DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae.
Nature. 2000 Aug 3;406(6795):477-83.
- PubMed ID
- 10952301 [ View in PubMed]
- Abstract
Here we determine the complete genomic sequence of the gram negative, gamma-Proteobacterium Vibrio cholerae El Tor N16961 to be 4,033,460 base pairs (bp). The genome consists of two circular chromosomes of 2,961,146 bp and 1,072,314 bp that together encode 3,885 open reading frames. The vast majority of recognizable genes for essential cell functions (such as DNA replication, transcription, translation and cell-wall biosynthesis) and pathogenicity (for example, toxins, surface antigens and adhesins) are located on the large chromosome. In contrast, the small chromosome contains a larger fraction (59%) of hypothetical genes compared with the large chromosome (42%), and also contains many more genes that appear to have origins other than the gamma-Proteobacteria. The small chromosome also carries a gene capture system (the integron island) and host 'addiction' genes that are typically found on plasmids; thus, the small chromosome may have originally been a megaplasmid that was captured by an ancestral Vibrio species. The V. cholerae genomic sequence provides a starting point for understanding how a free-living, environmental organism emerged to become a significant human bacterial pathogen.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID Cholera enterotoxin subunit B P01556 Details Uncharacterized protein Q9KVB4 Details DevB protein Q9KL51 Details Type II secretion system protein E P37093 Details Beta-hexosaminidase Q9KU37 Details 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Q9KPI8 Details CAI-1 autoinducer synthase Q9KM65 Details