Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis.
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Jenkins AL, Zhang Y, Ealick SE, Begley TP
Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis.
Bioorg Chem. 2008 Feb;36(1):29-32. Epub 2007 Dec 3.
- PubMed ID
- 18054064 [ View in PubMed]
- Abstract
TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here, we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each of the active site residues are identified and a mechanism for the reaction is described.