Protein complexes of the Escherichia coli cell envelope.
Article Details
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Stenberg F, Chovanec P, Maslen SL, Robinson CV, Ilag LL, von Heijne G, Daley DO
Protein complexes of the Escherichia coli cell envelope.
J Biol Chem. 2005 Oct 14;280(41):34409-19. Epub 2005 Aug 3.
- PubMed ID
- 16079137 [ View in PubMed]
- Abstract
Protein complexes are an intrinsic aspect of life in the membrane. Knowing which proteins are assembled in these complexes is therefore essential to understanding protein function(s). Unfortunately, recent high throughput protein interaction studies have failed to deliver any significant information on proteins embedded in the membrane, and many membrane protein complexes remain ill defined. In this study, we have optimized the blue native-PAGE technique for the study of membrane protein complexes in the inner and outer membranes of Escherichia coli. In combination with second dimension SDS-PAGE and mass spectrometry, we have been able to identify 43 distinct protein complexes. In addition to a number of well characterized complexes, we have identified known and orphan proteins in novel oligomeric states. For two orphan proteins, YhcB and YjdB, our findings enable a tentative functional assignment. We propose that YhcB is a hitherto unidentified additional subunit of the cytochrome bd oxidase and that YjdB, which co-localizes with the ZipA protein, is involved in cell division. Our reference two-dimensional blue native-SDS-polyacrylamide gels will facilitate future studies of the assembly and composition of E. coli membrane protein complexes during different growth conditions and in different mutant backgrounds.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID Outer membrane protein F P02931 Details Long-chain fatty acid transport protein P10384 Details Multidrug efflux pump subunit AcrB P31224 Details Outer membrane protein TolC P02930 Details ATP synthase subunit b P0ABA0 Details Outer membrane protein A P0A910 Details Maltoporin P02943 Details Succinate dehydrogenase flavoprotein subunit P0AC41 Details Succinate dehydrogenase iron-sulfur subunit P07014 Details Cell division protein ZipA P77173 Details