Dispersive liquid-liquid microextraction combined with ultra-high performance liquid chromatography for the simultaneous determination of 25 sulfonamide and quinolone antibiotics in water samples.

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Herrera-Herrera AV, Hernandez-Borges J, Borges-Miquel TM, Rodriguez-Delgado MA

Dispersive liquid-liquid microextraction combined with ultra-high performance liquid chromatography for the simultaneous determination of 25 sulfonamide and quinolone antibiotics in water samples.

J Pharm Biomed Anal. 2013 Mar 5;75:130-7. doi: 10.1016/j.jpba.2012.11.026. Epub 2012 Nov 23.

PubMed ID
23246932 [ View in PubMed
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Abstract

In this work, a dispersive liquid-liquid microextraction (DLLME) procedure combined with ultra-high performance liquid chromatography with diode-array detection was developed to determine 25 antibiotics in mineral and run-off waters. Optimum DLLME conditions (5 mL of water at pH=7.6, 20% (w/v) NaCl, 685 muL of CHCl(3) as extractant solvent, and 1250 muL of ACN as disperser solvent) allowed the repeatable, accurate and selective determination of 11 sulfonamides (sulfanilamide, sulfacetamide, sulfadiazine, sulfathiazole, sulfadimidin, sulfamethoxypyridazine, sulfadoxine, sulfamethoxazole, sulfisoxazole, sulfadimethoxine and sulfaquinoxaline) and 14 quinolones (pipemidic acid, marbofloxacin, fleroxacin, levofloxacin, pefloxacin, ciprofloxacin, lomefloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, moxifloxacin, oxolinic acid and flumequine). The method was validated by means of the obtention of calibration curves of the whole method as well as a recovery study at two levels of concentration. The LODs of the method were in the range 0.35-10.5 mug/L with recoveries between 78% and 117%.

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