Evaluation and validation of two microbiological tests for screening antibiotic residues in honey according to the European guideline for the validation of screening methods.

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Gaudin V, De Courville A, Hedou C, Rault A, Diomande SE, Creff-Froger C, Verdon E

Evaluation and validation of two microbiological tests for screening antibiotic residues in honey according to the European guideline for the validation of screening methods.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2013;30(2):234-43. doi: 10.1080/19440049.2012.738367. Epub 2012 Nov 6.

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23126529 [ View in PubMed
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Abstract

Two microbiological kits based on Bacillus stearothermophilus (Eclipse 50(R) and Premi(R)Test) have been evaluated and validated according to the European guideline for the validation of screening methods (January 2010) and in relation to the concentrations recommended by the EU-RL in 2007. Both tests are robust, a fast method and easy to implement. Both tests are applicable to a very large variety of honeys from different floral and geographical origins (rosemary, lavender, scrub, heath, alder, forest, lemon, acacia, chestnut, raspberry, mountain and flowers) as well as honey of different colours (from blank honey to brown honey, including yellow and orange honey). A satisfactory false-positive rate of 5% was obtained for the Eclipse 50(R) test. The observed detection capabilities CCbeta of the Eclipse 50(R) kit were: chlortetracycline (>75 microg kg(-1)), oxytetracycline (100 microg kg(-1)), cloxacillin (400 microg kg(-1)), sulfadiazine (300 microg kg(-1)), sulfamethazine (>1000 microg kg(-1)), sulfamethizole (>75 microg kg(-1)), sulfamethoxazole (>1000 microg kg(-1)), sulfaquinoxaline (>75 microg kg(-1)), sulfathiazole (1500 microg kg(-1)). These levels were all higher than the recommended concentrations where they exist. Due to its lack of sensitivity, it cannot be recommended for reliable routine use. The observed CCbeta of the Premi(R)Test kit were: chlortetracycline (10 microg kg(-1)), oxytetracycline (>10 microg kg(-1)), tetracycline (

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