Trapping and visualization of a covalent enzyme-phosphate intermediate.
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Murphy JE, Stec B, Ma L, Kantrowitz ER
Trapping and visualization of a covalent enzyme-phosphate intermediate.
Nat Struct Biol. 1997 Aug;4(8):618-22.
- PubMed ID
- 9253408 [ View in PubMed]
- Abstract
Using a mutant version of E. coli alkaline phosphatase, we succeeded in trapping and determining the structure of the phospho-enzyme intermediate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate.