Cloning and sequence analysis of the human gene encoding eosinophil major basic protein.
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Barker RL, Loegering DA, Arakawa KC, Pease LR, Gleich GJ
Cloning and sequence analysis of the human gene encoding eosinophil major basic protein.
Gene. 1990 Feb 14;86(2):285-9.
- PubMed ID
- 2323577 [ View in PubMed]
- Abstract
Eosinophil granule major basic protein (MBP), a potent toxin for helminths and mammalian cells, is a single polypeptide rich in arginine. The gene, mbp, was cloned and its nucleotide sequence determined. The 3.3-kb gene consists of six exons and five introns, one of which contains an Alu family repeat. The combined exon sequence is similar to previously reported mbp cDNA sequences. The gene is immediately preceded by a putative promoter containing typical TATA and CCAAT boxes. Southern blots indicate that mbp exhibits limited polymorphism.