Pro-major basic protein has three types of sugar chains at the pro-portion.
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Shikata Y, Hayashi Y, Yoshimatsu K, Ohya Y, Seto T, Fukushima K, Yoshida Y
Pro-major basic protein has three types of sugar chains at the pro-portion.
Biochim Biophys Acta. 1993 Jun 4;1163(3):243-9.
- PubMed ID
- 8507662 [ View in PubMed]
- Abstract
The amino-acid sequence of purified recombinant pro-major basic protein from Chinese hamster kidney cells was determined to verify the primary structure and glycosylation sites. Reduced and S-carboxamidemethylated protein was first digested with Achromobacter proteinase I. Each peptide was characterized by amino-acid analysis and amino-acid sequence analysis. We could identify all the peptides which were expected from the pro-major basic protein cDNA sequence. Sequence analysis and deglycosylation study revealed that Ser-8, Thr-9, Ser-46 and Asn-70 were glycosylated. The results indicated that proMBP has three types of sugar chains, O-glycoside, N-glycoside and glycosaminoglycan, in the pro-portion.