Location and nature of carbohydrate groups in proform of human major basic protein isolated from pregnancy serum.
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Oxvig C, Haaning J, Hojrup P, Sottrup-Jensen L
Location and nature of carbohydrate groups in proform of human major basic protein isolated from pregnancy serum.
Biochem Mol Biol Int. 1994 May;33(2):329-36.
- PubMed ID
- 7524900 [ View in PubMed]
- Abstract
From human pregnancy serum we have isolated the proform of eosinophil major basic protein (proMBP), which forms a complex with pregnancy-associated plasma protein-A (PAPP-A), PAPP-A/proMBP. It is shown that proMBP contains O-linked glycan bound to Ser-24, Thr-25 (fully substituted), and to Thr-23 and Thr-34 (partially substituted). N-linked glycan is bound to Asn-86 and O-linked glycosaminoglycan is bound to Ser-62 (both fully substituted). From the RP-HPLC elution profile and mass spectra of tryptic peptides it is found that proMBP is extremely heterogeneous with respect to glycosylation.