Aminopeptidase N
Details
- Name
- Aminopeptidase N
- Synonyms
- 3.4.11.2
- Alpha-aminoacylpeptide hydrolase
- Gene Name
- pepN
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0013024|Aminopeptidase N MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVRHGASDAPLRLNGEDLK LVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAANTALEGLYQSGDALCTQC EAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGNRVAQGELENGRHWVQWQDPF PKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRGNLDRAPWAMTSLKNSMKWDEER FGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYVLARTDTATDKDYLDIERVIGHEYF HNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDLGSRAVNRINNVRTMRGLQFAEDASPM AHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIHTLLGEENFQKGMQLYFERHDGSAATCDD FVQAMEDASNVDLSHFRRWYSQSGTPIVTVKDDYNPETEQYTLTISQRTPATPDQAEKQP LHIPFAIELYDNEGKVIPLQKGGHPVNSVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPV KLEYKWSDQQLTFLMRHARNDFSRWDAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFR AVLLDEKIDPALAAEILTLPSVNEMAELFDIIDPIAIAEVREALTRTLATELADELLAIY NANYQSEYRVEHEDIAKRTLRNACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAA VAAQLPCRDALMQEYDDKWHQNGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPN RIRSLIGAFAGSNPAAFHAEDGSGYLFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKR QEKMRAALEQLKGLENLSGDLYEKITKALA
- Number of residues
- 870
- Molecular Weight
- 98917.975
- Theoretical pI
- 4.95
- GO Classification
- Functionsaminopeptidase activity / identical protein binding / metallopeptidase activity / zinc ion bindingProcessespeptide catabolic processComponentsplasma membrane
- General Function
- Zinc ion binding
- Specific Function
- Aminopeptidase N is involved in the degradation of intracellular peptides generated by protein breakdown during normal growth as well as in response to nutrient starvation.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cell inner membrane
- Gene sequence
>lcl|BSEQ0013025|Aminopeptidase N (pepN) ATGACTCAACAGCCACAAGCCAAATACCGTCACGATTATCGTGCGCCGGATTACCAGATT ACTGATATTGACTTGACCTTTGACCTCGACGCGCAAAAGACGGTCGTTACCGCGGTCAGC CAGGCTGTCCGTCATGGTGCATCAGATGCTCCCCTTCGTCTCAACGGCGAAGACCTCAAA CTGGTTTCTGTTCATATTAATGATGAGCCGTGGACCGCCTGGAAAGAAGAAGAGGGCGCA CTGGTTATCAGTAATTTGCCGGAGCGTTTTACGCTTAAGATCATTAATGAAATAAGCCCG GCGGCGAATACCGCGCTGGAAGGGCTTTATCAGTCAGGCGATGCGCTTTGCACCCAGTGT GAAGCCGAAGGTTTCCGCCATATTACGTATTATCTCGACCGCCCGGACGTGCTGGCGCGT TTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAAC CGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTC CCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACC TTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTT GATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGC TTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATTTCTTCAATATG GGCGCAATGGAGAATAAGGGGCTGAATATCTTTAACTCCAAATATGTGCTGGCCCGCACC GACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTC CATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAA GGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAAC CGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATG GCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTT TACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTC CAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGAC TTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTAC AGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAG TACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCG CTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAG AAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTC TTTGATAATGTCTACTTCCAGCCGGTGCCTGCGCTGCTGTGCGAATTCTCTGCGCCAGTG AAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAAT GATTTCTCCCGCTGGGATGCGGCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTC GCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGC GCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAGAAATCCTGACGCTGCCT TCTGTCAATGAAATGGCTGAATTGTTCGATATCATCGACCCGATTGCTATTGCCGAAGTA CGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTAC AACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTG CGTAATGCCTGCCTGCGTTTCCTCGCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTG AGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCG GTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCAT CAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAAT GTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAAC CGTATTCGTTCGTTGATTGGCGCGTTTGCGGGCAGCAATCCGGCAGCGTTCCATGCCGAA GATGGCAGCGGTTACCTGTTCCTGGTGGAAATGCTTACCGACCTCAACAGCCGTAACCCG CAGGTGGCTTCACGTCTGATTGAACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGT CAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGAT CTGTACGAGAAGATAACTAAAGCACTGGCTTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P04825 UniProtKB Entry Name AMPN_ECOLI GenBank Protein ID 42353 GenBank Gene ID X04020 - General References
- Foglino M, Gharbi S, Lazdunski A: Nucleotide sequence of the pepN gene encoding aminopeptidase N of Escherichia coli. Gene. 1986;49(3):303-9. [Article]
- McCaman MT, Gabe JD: The nucleotide sequence of the pepN gene and its over-expression in Escherichia coli. Gene. 1986;48(1):145-53. [Article]
- Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- McCaman MT, Gabe JD: Sequence of the promoter and 5' coding region of pepN, and the amino-terminus of peptidase N from Escherichia coli K-12. Mol Gen Genet. 1986 Jul;204(1):148-52. [Article]
- Bally M, Foglino M, Bruschi M, Murgier M, Lazdunski A: Nucleotide sequence of the promoter and amino-terminal encoding region of the Escherichia coli pepN gene. Eur J Biochem. 1986 Mar 17;155(3):565-9. [Article]
- Ito K, Nakajima Y, Onohara Y, Takeo M, Nakashima K, Matsubara F, Ito T, Yoshimoto T: Crystal structure of aminopeptidase N (proteobacteria alanyl aminopeptidase) from Escherichia coli and conformational change of methionine 260 involved in substrate recognition. J Biol Chem. 2006 Nov 3;281(44):33664-76. Epub 2006 Aug 2. [Article]
- Addlagatta A, Gay L, Matthews BW: Structural basis for the unusual specificity of Escherichia coli aminopeptidase N. Biochemistry. 2008 May 13;47(19):5303-11. doi: 10.1021/bi7022333. Epub 2008 Apr 17. [Article]
- Fournie-Zaluski MC, Poras H, Roques BP, Nakajima Y, Ito K, Yoshimoto T: Structure of aminopeptidase N from Escherichia coli complexed with the transition-state analogue aminophosphinic inhibitor PL250. Acta Crystallogr D Biol Crystallogr. 2009 Aug;65(Pt 8):814-22. doi: 10.1107/S090744490901779X. Epub 2009 Jul 17. [Article]