Streptopain
Details
- Name
- Streptopain
- Synonyms
- 3.4.22.10
- Exotoxin type B
- SPE B
- SPP
- Streptococcal cysteine proteinase
- Streptococcus peptidase A
- Gene Name
- speB
- Organism
- Streptococcus pyogenes
- Amino acid sequence
>lcl|BSEQ0011460|Streptopain MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAITFIQKSAAIKAGARSAE DIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILGYSTSGSFDANGKENIASF MESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHYNQGNPYNLLTPVIEKVKPGE QSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTLSSNNPYFNHPKNLFAAISTRQY NWNNILPTYSGRESNVQKMAISELMADVGISVDMDYGPSSGSAGSSRVQRALKENFGYNQ SVHQINRSDFSKQDWEAQIDKELSQNQPVYYQGVGKVGGHAFVIDGADGRNFYHVNWGWG GVSDGFFRLDALNPSALGTGGGAGGFNGYQSAVVGIKP
- Number of residues
- 398
- Molecular Weight
- 43174.095
- Theoretical pI
- 9.11
- GO Classification
- Functionscysteine-type peptidase activityProcessespathogenesis / proteolysis in other organismComponentsextracellular region
- General Function
- Cysteine-type peptidase activity
- Specific Function
- Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Secreted
- Gene sequence
>lcl|BSEQ0004001|1197 bp ATGAATAAAAAGAAATTAGGTATCAGATTATTAAGTCTTTTAGCATTAGGTGGATTTGTT CTTGCTAACCCAGTATTTGCCGATCAAAACTTTGCTCGTAACGAAAAAGAAGCAAAAGAT AGCGCTATCACATTTATCCAAAAATCAGCAGCTATCAAAGCAGGTGCACGAAGCGCAGAA GATATTAAGCTTGACAAAGTTAACTTAGGTGGAGAACTTTCTGGCTCTAATATGTATGTT TACAATATTTCTACTGGAGGATTTGTTATCGTTTCAGGAGATAAACGTTCTCCAGAAATT CTAGGATACTCTACCAGCGGATCATTTGACGCTAACGGTAAAGAAAACATTGCTTCCTTC ATGGAAAGTTATGTCGAACAAATCAAAGAAAACAAAAAATTAGACACTACTTATGCTGGT ACCGCTGAGATTAAACAACCAGTTGTTAAATCTCTCCTTGATTCAAAAGGCATTCATTAC AACCAAGGTAACCCTTACAACCTATTGACACCTGTTATTGAAAAAGTAAAACCAGGTGAA CAATCTTTTGTAGGTCAACATGCAGCTACAGGATGTGTTGCTACTGCAACTGCTCAAATT ATGAAATATCATAATTACCCTAACAAAGGGTTGAAAGACTACACTTACACACTAAGCTCA AATAACCCATATTTCAACCATCCTAAGAACTTGTTTGCAGCTATCTCTACTAGACAATAC AACTGGAACAACATCCTACCTACTTATAGCGGAAGAGAATCTAACGTTCAAAAAATGGCG ATTTCAGAATTGATGGCTGATGTTGGTATTTCAGTAGACATGGATTATGGTCCATCTAGT GGTTCTGCAGGTAGCTCTCGTGTTCAAAGAGCCTTGAAAGAAAACTTTGGCTACAACCAA TCTGTTCACCAAATTAACCGTAGCGACTTTAGCAAACAAGATTGGGAAGCACAAATTGAC AAAGAATTATCTCAAAACCAACCAGTATACTACCAAGGTGTCGGTAAAGTAGGCGGACAT GCCTTTGTTATCGATGGTGCTGACGGACGTAACTTCTACCATGTTAACTGGGGTTGGGGT GGAGTCTCTGACGGCTTCTTCCGTCTTGACGCACTAAACCCTTCAGCTCTTGGTACTGGT GGCGGCGCAGGCGGCTTCAACGGTTACCAAAGTGCTGTTGTAGGCATCAAACCTTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0C0J0 UniProtKB Entry Name SPEB_STRPY GenBank Protein ID 153819 GenBank Gene ID M86905 - General References
- Hauser AR, Schlievert PM: Nucleotide sequence of the streptococcal pyrogenic exotoxin type B gene and relationship between the toxin and the streptococcal proteinase precursor. J Bacteriol. 1990 Aug;172(8):4536-42. [Article]
- Kapur V, Topouzis S, Majesky MW, Li LL, Hamrick MR, Hamill RJ, Patti JM, Musser JM: A conserved Streptococcus pyogenes extracellular cysteine protease cleaves human fibronectin and degrades vitronectin. Microb Pathog. 1993 Nov;15(5):327-46. [Article]
- Tai JY, Kortt AA, Liu TY, Elliott SD: Primary structure of streptococcal proteinase. III. Isolation of cyanogen bromide peptides: complete covalent structure of the polypeptide chain. J Biol Chem. 1976 Apr 10;251(7):1955-9. [Article]
- Lo SS, Fraser BA, Liu TY: The mixed disulfide in the zymogen of streptococcal proteinase. Characterization and implication for its biosynthesis. J Biol Chem. 1984 Sep 10;259(17):11041-5. [Article]
- Kuo CF, Wu JJ, Tsai PJ, Kao FJ, Lei HY, Lin MT, Lin YS: Streptococcal pyrogenic exotoxin B induces apoptosis and reduces phagocytic activity in U937 cells. Infect Immun. 1999 Jan;67(1):126-30. [Article]
- Kagawa TF, Cooney JC, Baker HM, McSweeney S, Liu M, Gubba S, Musser JM, Baker EN: Crystal structure of the zymogen form of the group A Streptococcus virulence factor SpeB: an integrin-binding cysteine protease. Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2235-40. [Article]
- Olsen JG, Dagil R, Niclasen LM, Sorensen OE, Kragelund BB: Structure of the mature Streptococcal cysteine protease exotoxin mSpeB in its active dimeric form. J Mol Biol. 2009 Oct 30;393(3):693-703. doi: 10.1016/j.jmb.2009.08.046. Epub 2009 Aug 25. [Article]