Mutagenesis of the gene encoding amicyanin of Paracoccus denitrificans and the resultant effect on methylamine oxidation.
Article Details
- CitationCopy to clipboard
van Spanning RJ, Wansell CW, Reijnders WN, Oltmann LF, Stouthamer AH
Mutagenesis of the gene encoding amicyanin of Paracoccus denitrificans and the resultant effect on methylamine oxidation.
FEBS Lett. 1990 Nov 26;275(1-2):217-20.
- PubMed ID
- 2261991 [ View in PubMed]
- Abstract
The gene encoding the blue-copper protein amicyanin was isolated from a genomic bank of Paracoccus denitrificans by using a synthetic oligonucleotide. It is located directly downstream of the gene encoding the small subunit of methylamine dehydrogenase. Amicyanin is transcribed as a precursor protein with a signal sequence, typical for periplasmic proteins. Specific inactivation of amicyanin by means of gene replacement techniques resulted in the complete loss of the ability to grow on methylamine.