Analysis of binding interaction between antibacterial ciprofloxacin and human serum albumin by spectroscopic techniques.
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Varshney A, Ansari Y, Zaidi N, Ahmad E, Badr G, Alam P, Khan RH
Analysis of binding interaction between antibacterial ciprofloxacin and human serum albumin by spectroscopic techniques.
Cell Biochem Biophys. 2014 Sep;70(1):93-101. doi: 10.1007/s12013-014-9863-1.
- PubMed ID
- 24913236 [ View in PubMed]
- Abstract
The binding of ciprofloxacin (CFX) to human serum albumin (HSA) has been investigated by fluorescence displacement and induced circular dichroism (ICD) measurements. Displacement measurements were performed with CFX in the absence and presence of marker ligands (hemin for domain I, bilirubin for interspace of domain IA and IIA, chloroform for domain II, and diazepam for domain III) to establish CFX binding site in one of the three major domains of HSA. The primary binding site of CFX is located in site I of HSA (domain IIA) in close vicinity to the site where chloroform (CHCl3) binds. It is depicted from the decrease in quenching constant of HSA-CHCl3 system (0.02 +/- 0.06) x 10(-3 )L mol(-1) compared to HSA-CFX-CHCl3 system (0.01 +/- 0.06) x 10(-3 )L mol(-1) as obtained by the fluorescence displacement spectroscopy. Furthermore, far-UV CD results show that the binding of CFX leads to change in the helicity of HSA. The ICD results indicated that the CFX binds to the domain IIA of HSA which is in agreement with the fluorescence displacement results.
DrugBank Data that Cites this Article
- Drugs
- Drug Carriers
Drug Carrier Kind Organism Pharmacological Action Actions Ciprofloxacin Serum albumin Protein Humans UnknownBinderDetails