inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis.

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Banerjee A, Dubnau E, Quemard A, Balasubramanian V, Um KS, Wilson T, Collins D, de Lisle G, Jacobs WR Jr

inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis.

Science. 1994 Jan 14;263(5144):227-30.

PubMed ID

8284673 [ View in PubMed

]

Abstract

Isoniazid (isonicotinic acid hydrazide, INH) is one of the most widely used antituberculosis drugs, yet its precise target of action on Mycobacterium tuberculosis is unknown. A missense mutation within the mycobacterial inhA gene was shown to confer resistance to both INH and ethionamide (ETH) in M. smegmatis and in M. bovis. The wild-type inhA gene also conferred INH and ETH resistance when transferred on a multicopy plasmid vector to M. smegmatis and M. bovis BCG. The InhA protein shows significant sequence conservation with the Escherichia coli enzyme EnvM, and cell-free assays indicate that it may be involved in mycolic acid biosynthesis. These results suggest that InhA is likely a primary target of action for INH and ETH.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Ethionamide	Enoyl-[acyl-carrier-protein] reductase [NADH]	Protein	Mycobacterium tuberculosis	Yes	Inhibitor	Details

Polypeptides

Name	UniProt ID
Enoyl-[acyl-carrier-protein] reductase [NADH]	P9WGR1	Details