Crystal structure of the ribosome at 5.5 A resolution.
Article Details
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Yusupov MM, Yusupova GZ, Baucom A, Lieberman K, Earnest TN, Cate JH, Noller HF
Crystal structure of the ribosome at 5.5 A resolution.
Science. 2001 May 4;292(5518):883-96. Epub 2001 Mar 29.
- PubMed ID
- 11283358 [ View in PubMed]
- Abstract
We describe the crystal structure of the complete Thermus thermophilus 70S ribosome containing bound messenger RNA and transfer RNAs (tRNAs) at 5.5 angstrom resolution. All of the 16S, 23S, and 5S ribosomal RNA (rRNA) chains, the A-, P-, and E-site tRNAs, and most of the ribosomal proteins can be fitted to the electron density map. The core of the interface between the 30S small subunit and the 50S large subunit, where the tRNA substrates are bound, is dominated by RNA, with proteins located mainly at the periphery, consistent with ribosomal function being based on rRNA. In each of the three tRNA binding sites, the ribosome contacts all of the major elements of tRNA, providing an explanation for the conservation of tRNA structure. The tRNAs are closely juxtaposed with the intersubunit bridges, in a way that suggests coupling of the 20 to 50 angstrom movements associated with tRNA translocation with intersubunit movement.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID 50S ribosomal protein L27 P60493 Details 30S ribosomal protein S10 Q5SHN7 Details 30S ribosomal protein S12 Q5SHN3 Details 30S ribosomal protein S11 P80376 Details 30S ribosomal protein S13 P80377 Details 30S ribosomal protein S15 Q5SJ76 Details 30S ribosomal protein S16 Q5SJH3 Details 30S ribosomal protein S18 Q5SLQ0 Details 30S ribosomal protein S19 Q5SHP2 Details 30S ribosomal protein S20 P80380 Details 30S ribosomal protein S4 P80373 Details 30S ribosomal protein S5 Q5SHQ5 Details 30S ribosomal protein S6 Q5SLP8 Details 30S ribosomal protein S7 P17291 Details 30S ribosomal protein S9 P80374 Details 30S ribosomal protein Thx Q5SIH3 Details 30S ribosomal protein S2 P80371 Details 30S ribosomal protein S3 P80372 Details 30S ribosomal protein S14 type Z P0DOY6 Details 30S ribosomal protein S17 P0DOY7 Details 30S ribosomal protein S8 P0DOY9 Details