CENP-E kinesin interacts with SKAP protein to orchestrate accurate chromosome segregation in mitosis.
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Huang Y, Wang W, Yao P, Wang X, Liu X, Zhuang X, Yan F, Zhou J, Du J, Ward T, Zou H, Zhang J, Fang G, Ding X, Dou Z, Yao X
CENP-E kinesin interacts with SKAP protein to orchestrate accurate chromosome segregation in mitosis.
J Biol Chem. 2012 Jan 6;287(2):1500-9. doi: 10.1074/jbc.M111.277194. Epub 2011 Nov 22.
- PubMed ID
- 22110139 [ View in PubMed]
- Abstract
Mitotic chromosome segregation is orchestrated by the dynamic interaction of spindle microtubules with the kinetochore. Although previous studies show that the mitotic kinesin CENP-E forms a link between attachment of the spindle microtubule to the kinetochore and the mitotic checkpoint signaling cascade, the molecular mechanism underlying dynamic kinetochore-microtubule interactions in mammalian cells remains elusive. Here, we identify a novel interaction between CENP-E and SKAP that functions synergistically in governing dynamic kinetochore-microtubule interactions. SKAP binds to the C-terminal tail of CENP-E in vitro and is essential for an accurate kinetochore-microtubule attachment in vivo. Immunoelectron microscopic analysis indicates that SKAP is a constituent of the kinetochore corona fibers of mammalian centromeres. Depletion of SKAP or CENP-E by RNA interference results in a dramatic reduction of inter-kinetochore tension, which causes chromosome mis-segregation with a prolonged delay in achieving metaphase alignment. Importantly, SKAP binds to microtubules in vitro, and this interaction is synergized by CENP-E. Based on these findings, we propose that SKAP cooperates with CENP-E to orchestrate dynamic kinetochore-microtubule interaction for faithful chromosome segregation.