Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3.
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Pioletti M, Schlunzen F, Harms J, Zarivach R, Gluhmann M, Avila H, Bashan A, Bartels H, Auerbach T, Jacobi C, Hartsch T, Yonath A, Franceschi F
Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3.
EMBO J. 2001 Apr 17;20(8):1829-39.
- PubMed ID
- 11296217 [ View in PubMed]
- Abstract
The small ribosomal subunit is responsible for the decoding of genetic information and plays a key role in the initiation of protein synthesis. We analyzed by X-ray crystallography the structures of three different complexes of the small ribosomal subunit of Thermus thermophilus with the A-site inhibitor tetracycline, the universal initiation inhibitor edeine and the C-terminal domain of the translation initiation factor IF3. The crystal structure analysis of the complex with tetracycline revealed the functionally important site responsible for the blockage of the A-site. Five additional tetracycline sites resolve most of the controversial biochemical data on the location of tetracycline. The interaction of edeine with the small subunit indicates its role in inhibiting initiation and shows its involvement with P-site tRNA. The location of the C-terminal domain of IF3, at the solvent side of the platform, sheds light on the formation of the initiation complex, and implies that the anti-association activity of IF3 is due to its influence on the conformational dynamics of the small ribosomal subunit.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID 30S ribosomal protein S10 Q5SHN7 Details 30S ribosomal protein S12 Q5SHN3 Details 30S ribosomal protein S11 P80376 Details 30S ribosomal protein S13 P80377 Details 30S ribosomal protein S15 Q5SJ76 Details 30S ribosomal protein S16 Q5SJH3 Details 30S ribosomal protein S18 Q5SLQ0 Details 30S ribosomal protein S19 Q5SHP2 Details 30S ribosomal protein S20 P80380 Details 30S ribosomal protein S4 P80373 Details 30S ribosomal protein S5 Q5SHQ5 Details 30S ribosomal protein S6 Q5SLP8 Details 30S ribosomal protein S7 P17291 Details 30S ribosomal protein S9 P80374 Details 30S ribosomal protein Thx Q5SIH3 Details 30S ribosomal protein S2 P62662 Details 30S ribosomal protein S3 P62663 Details 30S ribosomal protein S9 P62669 Details 30S ribosomal protein S2 P80371 Details 30S ribosomal protein S3 P80372 Details 30S ribosomal protein S14 type Z P0DOY6 Details 30S ribosomal protein S17 P0DOY7 Details 30S ribosomal protein S8 P0DOY9 Details