The biochemical pharmacology of (2'-R)-chloropentostatin, a novel inhibitor of adenosine deaminase.
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Jackson RC, Leopold WR, Ross DA
The biochemical pharmacology of (2'-R)-chloropentostatin, a novel inhibitor of adenosine deaminase.
Adv Enzyme Regul. 1986;25:125-39.
- PubMed ID
- 2433905 [ View in PubMed]
- Abstract
2'-Chloropentostatin is a new inhibitor of adenosine deaminase isolated from the fermentation broth of an unidentified actinomycete, ATCC 39365. It contains the aglycone of coformycin, i.e. 3,6,7,8-tetrahydroimidazo[4,5-d][1,3]diazepin-8-o1, coupled to the unusual carbohydrate, 2'-chloro-2'-deoxyribose. 2'-Chloropentostatin is a slightly weaker inhibitor of rat and human adenosine deaminases than coformycin, and considerably weaker than pentostatin. Unlike pentostatin, which appears to undergo a two-stage interaction with adenosine deaminase, 2'-chloropentostatin forms a single enzyme-inhibitor complex. The enzyme-inhibitor complex between adenosine deaminase and 2'-chloropentostatin was much more rapidly dissociable than the complex with pentostatin. The complex between adenosine deaminase and 2'-chloropentostatin dissociated with a half-life of approximately 3 hr, compared with 68 hr for the complex between adenosine deaminase and pentostatin. 2'-Chloropentostatin, at concentrations up to 10 micromolar, did not cause significant inhibition of growth of WI-L2 human B-cell lymphoblasts, or of CCRF-CEM human T-cell lymphoblasts in culture. However, it greatly potentiated the inhibitory potency of adenosine, 2'-deoxyadenosine, or arabinosyladenine towards these cell lines. This potentiating effect was equipotent for 2'-chloropentostatin and pentostatin. T-cells (CCRF-CEM) were much more sensitive to the inhibitory effect of combinations of adenosine or 2'-deoxyadenosine with 2'-chloropentostatin or pentostatin than were B-cells (WI-L2). Pentostatin and 2'-chloropentostatin had no significant antitumor activity against mouse leukemia L1210 in vivo. However, these adenosine deaminase inhibitors, at nontoxic doses, greatly potentiated the antitumor activity of ara-A 5'-phosphate. 2'-Chloropentostatin was somewhat more active in this regard than was pentostatin.
DrugBank Data that Cites this Article
- Drug Targets
Drug Target Kind Organism Pharmacological Action Actions Pentostatin Adenosine deaminase Protein Humans YesInhibitorDetails