Mechanism of action of bacitracin: complexation with metal ion and C 55 -isoprenyl pyrophosphate.

Article Details

Citation

Copy to clipboard

Stone KJ, Strominger JL

Mechanism of action of bacitracin: complexation with metal ion and C 55 -isoprenyl pyrophosphate.

Proc Natl Acad Sci U S A. 1971 Dec;68(12):3223-7.

PubMed ID

4332017 [ View in PubMed

]

Abstract

The inhibition by bacitracin of the enzymatic dephosphorylation of C(55)-isoprenyl pyrophosphate is abolished by the addition of chelating agents. If, however, the chelating agent is added after a preincubation of bacitracin with a divalent cation and the lipid substrate, then its addition has little effect, indicating that bacitracin, metal ion, and C(55)-isoprenyl pyrophosphate form a complex. Various divalent cations can participate in complex formation, but monovalent cations are ineffective. A direct demonstration of the formation of a complex between the C(55)-isoprenyl pyrophosphate and bacitracin in the presence of metal ions was obtained. Molecular models that show one possible conformation for a complex between bacitracin and the C(55)-isoprenyl pyrophosphate, in which the metal ion acts as a bridge between the two compounds, are presented.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Bacitracin	C55-isoprenyl pyrophosphate	Small molecule	Gram positive and gram negative bacteria	Yes	Antagonist	Details