Spectrofluorometric analysis of CYP2A6-catalyzed coumarin 7-hydroxylation.
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Waxman DJ, Chang TK
Spectrofluorometric analysis of CYP2A6-catalyzed coumarin 7-hydroxylation.
Methods Mol Biol. 2006;320:91-6. doi: 10.1385/1-59259-998-2:91.
- PubMed ID
- 16719377 [ View in PubMed]
- Abstract
A spectrofluorometric method is described for the determination of CYP2A6-catalyzed coumarin 7-hydroxylation. Following acidification of the reaction mixture, the enzymatic product, 7-hydroxycoumarin, is recovered by a double-extraction procedure and assayed using an excitation wavelength of 370 nm and an emission wavelength of 450 nm. This assay is applicable to enzymatic studies of cDNA-expressed CYP2A6 and can be used to monitor coumarin 7-hydroxylation activity in microsomes prepared from liver and other tissues and in isolated hepatocytes and cultured cells that express this cytochrome P450 activity.
DrugBank Data that Cites this Article
- Drug Enzymes
Drug Enzyme Kind Organism Pharmacological Action Actions Dexamethasone Cytochrome P450 2A6 Protein Humans UnknownInducerDetails Dexamethasone acetate Cytochrome P450 2A6 Protein Humans UnknownInducerDetails