High sensitivity methods to quantify chloroquine and its metabolite in human blood samples using LC-MS/MS.
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Kaewkhao K, Chotivanich K, Winterberg M, Day NP, Tarning J, Blessborn D
High sensitivity methods to quantify chloroquine and its metabolite in human blood samples using LC-MS/MS.
Bioanalysis. 2019 Mar;11(5):333-347. doi: 10.4155/bio-2018-0202. Epub 2019 Mar 15.
- PubMed ID
- 30873854 [ View in PubMed]
- Abstract
AIM: Chloroquine is an antimalarial drug used in the treatment of Plasmodium vivax malaria. Three methods to quantify chloroquine and its metabolite in blood matrices were developed and validated. Methodology & results: Different high-throughput extraction techniques were used to recover the drugs from whole blood (50 mul), plasma (100 mul) and dried blood spots (15 mul as punched discs) followed by quantification with LC-MS/MS. The intra- and inter-batch precisions were below 15%, and thus meet regulatory acceptance criteria. CONCLUSION: The developed methods demonstrated satisfactory validation performance with high sensitivity and selectivity. The assays used simple and easy to automate extraction techniques. All methods were reliable with robust performance and demonstrated to be suitable to implement into high-throughput routine analysis of clinical pharmacokinetic samples.
DrugBank Data that Cites this Article
- Drugs
- Drug Enzymes
Drug Enzyme Kind Organism Pharmacological Action Actions Chloroquine Cytochrome P450 2C8 Protein Humans UnknownSubstrateDetails Chloroquine Cytochrome P450 3A4 Protein Humans UnknownSubstrateDetails Chloroquine Cytochrome P450 3A5 Protein Humans NoSubstrateDetails - Drug Reactions
Reaction Details
