Drug-human serum albumin binding studied by capillary electrophoresis with electrochemiluminescence detection.
Article Details
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Zhao X, You T, Liu J, Sun X, Yan J, Yang X, Wang E
Drug-human serum albumin binding studied by capillary electrophoresis with electrochemiluminescence detection.
Electrophoresis. 2004 Oct;25(20):3422-6. doi: 10.1002/elps.200305930.
- PubMed ID
- 15490448 [ View in PubMed]
- Abstract
A new technique for investigating drug-protein binding was developed employing capillary electrophoresis (CE) coupled with tris(2,2'-bipyridyl) ruthenium(II) [Ru(bpy)(3) (2+)] electrochemiluminescence (ECL) (CE-ECL) detection after equilibrium dialysis. Three basic drugs, namely pridinol, procyclidine and its analogue trihexyphenidyl, were successfully separated by capillary zone electrophoresis with end-column Ru(bpy)(3) (2+) ECL detection. The relative drug binding to human serum albumin (HSA) for each single drug as well as for the three drugs binding simultaneously was calculated. It was found that the three antiparkinsonian drugs compete for the same binding site on HSA. This work demonstrated that Ru(bpy)(3) (2+) CE-ECL can be a suitable technique for studying drug-protein binding.
DrugBank Data that Cites this Article
- Drugs
- Drug Carriers
Drug Carrier Kind Organism Pharmacological Action Actions Trihexyphenidyl Serum albumin Protein Humans UnknownBinderDetails