Determination of tryptophan tRNA recognition sites for tryptophanyl-tRNA synthetase from hyperthermophilic archaeon, Aeropyrum pernix K1.

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Tsuchiya W, Umehara T, Kuno A, Hasegawa T

Determination of tryptophan tRNA recognition sites for tryptophanyl-tRNA synthetase from hyperthermophilic archaeon, Aeropyrum pernix K1.

Nucleic Acids Symp Ser (Oxf). 2004;(48):185-6.

PubMed ID

17150540 [ View in PubMed

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Abstract

To investigate the recognition mechanism of tryptophan tRNA by tryptophanyl-tRNA synthetase from extreme hyperthermophilic and aerobic archaeon, Aeropyrum pernix K1, tryptophanylation activities were examined by using mutant tryptophan tRNA transcripts prepared by in vitro transcription system. Their transcripts were aminoacylated with tryptophan by overexpressed A. pernix tryptophanyl-tRNA synthetase. The results indicated that anticodon nucleotides C34, C35 and A36, discriminator base A73, G1-C72 and G2-C71 base pairs of acceptor stem were base-specifically recognized by A. pernix tryptophanyl-tRNA synthetase.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Tryptophan	Tryptophan--tRNA ligase	Protein	Geobacillus stearothermophilus	Unknown	Inhibitor	Details
Tryptophan	Tryptophan--tRNA ligase, cytoplasmic	Protein	Humans	Unknown	Inhibitor	Details

Drug Enzymes

Drug	Enzyme	Kind	Organism	Pharmacological Action	Actions
Tryptophan	Tryptophan--tRNA ligase	Protein	Geobacillus stearothermophilus	Unknown	Substrate Inhibitor	Details
Tryptophan	Tryptophan--tRNA ligase, cytoplasmic	Protein	Humans	Unknown	Substrate Inhibitor	Details