(2Z,6E)-farnesyl diphosphate synthase
Details
- Name
- (2Z,6E)-farnesyl diphosphate synthase
- Synonyms
- 2.5.1.68
- Short-chain Z-isoprenyl diphosphate synthase
- Z-FPP synthase
- Z-FPPS
- Z-isoprenyl diphosphate synthase
- Z-Polyprenyl diphosphate synthase
- Gene Name
- Not Available
- Organism
- Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
- Amino acid sequence
>lcl|BSEQ0051237|(2Z,6E)-farnesyl diphosphate synthase MEIIPPRLKEPLYRLYELRLRQGLAASKSDLPRHIAVLCDGNRRWARSAGYDDVSYGYRM GAAKIAEMLRWCHEAGIELATVYLLSTENLQRDPDELAALIEIITDVVEEICAPANHWSV RTVGDLGLIGEEPARRLRGAVESTPEVASFHVNVAVGYGGRREIVDAVRALLSKELANGA TAEELVDAVTVEGISENLYTSGQPDPDLVIRTSGEQRLSGFLLWQSAYSEMWFTEAHWPA FRHVDFLRALRDYSARHRSYGR
- Number of residues
- 262
- Molecular Weight
- 29409.955
- Theoretical pI
- Not Available
- GO Classification
- Functionsmagnesium ion binding / manganese ion binding / polyprenyltransferase activity / Z-farnesyl diphosphate synthase activityProcessespolyprenol biosynthetic processComponentscytosol / plasma membrane
- General Function
- Catalyzes the condensation of only one isopentenyl pyrophosphate (IPP) unit in the cis configuration to E-geranyl diphosphate (E-GPP) generating the 15 carbon product (2Z,6E)-farnesyl diphosphate (Z-FPP or EZ-FPP). Z-FPP is the precursor of decaprenyl diphosphate, which has a central role in the biosynthesis of the mycobacterial cell wall.
- Specific Function
- Magnesium ion binding
- Pfam Domain Function
- Prenyltransf (PF01255)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0051238|(2Z,6E)-farnesyl diphosphate synthase GTGGAGATCATCCCGCCGCGGCTCAAAGAGCCGTTGTACCGGCTCTACGAGCTGCGCCTG CGGCAGGGCTTGGCCGCCTCGAAATCCGACCTGCCCCGGCACATAGCCGTGCTGTGCGAC GGCAACCGGCGATGGGCGCGCAGCGCGGGCTACGACGACGTCAGCTACGGCTACCGGATG GGTGCGGCCAAGATCGCCGAAATGCTGCGGTGGTGCCACGAAGCCGGCATCGAACTGGCC ACCGTCTATCTGCTGTCCACCGAAAACCTGCAGCGCGATCCCGACGAGCTTGCAGCACTC ATCGAGATCATCACCGATGTCGTGGAAGAGATCTGCGCACCGGCCAACCACTGGAGTGTG CGGACGGTCGGGGATCTGGGGTTGATCGGCGAGGAACCGGCCCGGCGGCTGCGCGGTGCG GTGGAATCCACCCCGGAGGTGGCCTCGTTTCATGTCAACGTTGCTGTTGGCTACGGCGGG CGCCGCGAGATCGTCGACGCTGTGCGCGCGTTGTTGAGCAAGGAACTCGCCAACGGGGCC ACCGCGGAGGAACTCGTCGACGCGGTGACCGTCGAGGGTATCTCGGAAAACCTGTACACC TCAGGCCAACCCGACCCCGATTTGGTGATACGCACCTCCGGCGAGCAACGCTTGTCCGGG TTCTTGCTGTGGCAAAGCGCCTACTCGGAGATGTGGTTCACCGAGGCGCACTGGCCGGCG TTTCGCCACGTCGATTTTCTACGCGCGCTGCGTGACTACAGTGCGAGGCATCGCAGCTAC GGCAGGTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P9WFF5 UniProtKB Entry Name ZFPP_MYCTU - General References
- Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE 3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Krogh A, McLean J, Moule S, Murphy L, Oliver K, Osborne J, Quail MA, Rajandream MA, Rogers J, Rutter S, Seeger K, Skelton J, Squares R, Squares S, Sulston JE, Taylor K, Whitehead S, Barrell BG: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 1998 Jun 11;393(6685):537-44. [Article]
- Crick DC, Schulbach MC, Zink EE, Macchia M, Barontini S, Besra GS, Brennan PJ: Polyprenyl phosphate biosynthesis in Mycobacterium tuberculosis and Mycobacterium smegmatis. J Bacteriol. 2000 Oct;182(20):5771-8. [Article]
- Schulbach MC, Brennan PJ, Crick DC: Identification of a short (C15) chain Z-isoprenyl diphosphate synthase and a homologous long (C50) chain isoprenyl diphosphate synthase in Mycobacterium tuberculosis. J Biol Chem. 2000 Jul 28;275(30):22876-81. [Article]
- Schulbach MC, Mahapatra S, Macchia M, Barontini S, Papi C, Minutolo F, Bertini S, Brennan PJ, Crick DC: Purification, enzymatic characterization, and inhibition of the Z-farnesyl diphosphate synthase from Mycobacterium tuberculosis. J Biol Chem. 2001 Apr 13;276(15):11624-30. Epub 2001 Jan 4. [Article]
- Noike M, Ambo T, Kikuchi S, Suzuki T, Yamashita S, Takahashi S, Kurokawa H, Mahapatra S, Crick DC, Koyama T: Product chain-length determination mechanism of Z,E-farnesyl diphosphate synthase. Biochem Biophys Res Commun. 2008 Dec 5;377(1):17-22. doi: 10.1016/j.bbrc.2008.09.014. Epub 2008 Sep 13. [Article]
- Kelkar DS, Kumar D, Kumar P, Balakrishnan L, Muthusamy B, Yadav AK, Shrivastava P, Marimuthu A, Anand S, Sundaram H, Kingsbury R, Harsha HC, Nair B, Prasad TS, Chauhan DS, Katoch K, Katoch VM, Kumar P, Chaerkady R, Ramachandran S, Dash D, Pandey A: Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3. [Article]
- Wang W, Dong C, McNeil M, Kaur D, Mahapatra S, Crick DC, Naismith JH: The structural basis of chain length control in Rv1086. J Mol Biol. 2008 Aug 1;381(1):129-40. doi: 10.1016/j.jmb.2008.05.060. Epub 2008 Jul 1. [Article]