Use of a surface plasmon resonance method to investigate antibiotic and plasma protein interactions.
Article Details
- CitationCopy to clipboard
Baneres-Roquet F, Gualtieri M, Villain-Guillot P, Pugniere M, Leonetti JP
Use of a surface plasmon resonance method to investigate antibiotic and plasma protein interactions.
Antimicrob Agents Chemother. 2009 Apr;53(4):1528-31. doi: 10.1128/AAC.00971-08. Epub 2009 Jan 21.
- PubMed ID
- 19164148 [ View in PubMed]
- Abstract
The pharmacologic effect of an antibiotic is directly related to its unbound concentration at the site of infection. Most commercial antibiotics have been selected in part for their low propensity to interact with serum proteins. These nonspecific interactions are classically evaluated by measuring the MIC in the presence of serum. As higher-throughput technologies tend to lose information, surface plasmon resonance (SPR) is emerging as an informative medium-throughput technology for hit validation. Here we show that SPR is a useful automatic tool for quantification of the interaction of model antibiotics with serum proteins and that it delivers precise real-time kinetic data on this critical parameter.
DrugBank Data that Cites this Article
- Drug Targets
Drug Target Kind Organism Pharmacological Action Actions Cefotaxime Serum albumin Protein Humans UnknownNot Available Details - Drug Carriers
Drug Carrier Kind Organism Pharmacological Action Actions Rifampicin alpha1-acid glycoprotein (Protein Group) Protein group Humans UnknownLigandDetails Rifampicin Serum albumin Protein Humans UnknownLigandDetails Vancomycin alpha1-acid glycoprotein (Protein Group) Protein group Humans NoSubstrateDetails Vancomycin Histamine H1 receptor Protein Mouse NoSubstrateDetails Vancomycin Serum albumin Protein Humans NoSubstrateDetails - Binding Properties
Drug Target Property Measurement pH Temperature (°C) Cefotaxime Serum albumin Kd (nM) 12000 N/A N/A Details Vancomycin Serum albumin Kd (nM) 12000 N/A N/A Details