Effect of methanol, ethanol, dimethyl sulfoxide, and acetonitrile on in vitro activities of cDNA-expressed human cytochromes P-450.
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Busby WF Jr, Ackermann JM, Crespi CL
Effect of methanol, ethanol, dimethyl sulfoxide, and acetonitrile on in vitro activities of cDNA-expressed human cytochromes P-450.
Drug Metab Dispos. 1999 Feb;27(2):246-9.
- PubMed ID
- 9929510 [ View in PubMed]
- Abstract
The effects of methanol, ethanol, dimethyl sulfoxide (DMSO), and acetonitrile were studied in vitro on nine individual, cDNAexpressed cytochrome P-450 activities (phenacetin O-deethylase for CYP1A1 and CYP1A2, coumarin 7-hydroxylase for CYP2A6, testosterone 6beta-hydroxylase for CYP3A4, 7-ethoxy-4-trifluoromethylcoumarin deethylase for CYP2B6, paclitaxel 6alpha-hydroxylase for CYP2C8, diclofenac 4'-hydroxylase for CYP2C9, S-mephenytoin 4-hydroxylase for CYP2C19, and (+/-)-bufuralol 1'-hydroxylase for CYP2D6) in commercially available human lymphoblastoid microsomes. These data show that specific solvents have enzyme-selective effects on P-450 activities. Methanol did not substantially inhibit (
DrugBank Data that Cites this Article
- Drug Enzymes
Drug Enzyme Kind Organism Pharmacological Action Actions Dimethyl sulfoxide Cytochrome P450 2C19 Protein Humans UnknownInhibitorDetails Dimethyl sulfoxide Cytochrome P450 2D6 Protein Humans UnknownInhibitorDetails Dimethyl sulfoxide Cytochrome P450 3A4 Protein Humans UnknownInhibitorDetails Ethanol Cytochrome P450 1A1 Protein Humans UnknownInhibitorDetails Ethanol Cytochrome P450 2B6 Protein Humans UnknownInhibitorDetails Ethanol Cytochrome P450 2C19 Protein Humans UnknownInhibitorDetails Ethanol Cytochrome P450 3A4 Protein Humans UnknownSubstrateInhibitorInducerDetails