Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.
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Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD, Chen JL
Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.
Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9543-8.
- PubMed ID
- 10931946 [ View in PubMed]
- Abstract
The primary neuroendocrine interface, hypothalamus and pituitary, together with adrenals, constitute the major axis responsible for the maintenance of homeostasis and the response to the perturbations in the environment. The gene expression profiling in the human hypothalamus-pituitary-adrenal axis was catalogued by generating a large amount of expressed sequence tags (ESTs), followed by bioinformatics analysis (http://www.chgc.sh.cn/ database). Totally, 25,973 sequences of good quality were obtained from 31,130 clones (83.4%) from cDNA libraries of the hypothalamus, pituitary, and adrenal glands. After eliminating 5,347 sequences corresponding to repetitive elements and mtDNA, 20,626 ESTs could be assembled into 9, 175 clusters (3,979, 3,074, and 4,116 clusters in hypothalamus, pituitary, and adrenal glands, respectively) when overlapping ESTs were integrated. Of these clusters, 2,777 (30.3%) corresponded to known genes, 4,165 (44.8%) to dbESTs, and 2,233 (24.3%) to novel ESTs. The gene expression profiles reflected well the functional characteristics of the three levels in the hypothalamus-pituitary-adrenal axis, because most of the 20 genes with highest expression showed statistical difference in terms of tissue distribution, including a group of tissue-specific functional markers. Meanwhile, some findings were made with regard to the physiology of the axis, and 200 full-length cDNAs of novel genes were cloned and sequenced. All of these data may contribute to the understanding of the neuroendocrine regulation of human life.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial O75489 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2 Q9NRX3 Details NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial P49821 Details NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 P17568 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Q9P0J0 Details 3-oxo-5-alpha-steroid 4-dehydrogenase 1 P18405 Details UMP-CMP kinase P30085 Details NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Q9UI09 Details Peroxiredoxin-5, mitochondrial P30044 Details Coatomer subunit gamma-1 Q9Y678 Details S-formylglutathione hydrolase P10768 Details Peroxisomal sarcosine oxidase Q9P0Z9 Details ADP-ribosylation factor-like protein 5A Q9Y689 Details V-type proton ATPase catalytic subunit A P38606 Details Geranylgeranyl pyrophosphate synthase O95749 Details Enolase-phosphatase E1 Q9UHY7 Details Acyl-coenzyme A thioesterase 13 Q9NPJ3 Details Diphthine methyl ester synthase Q9H2P9 Details tRNA-splicing ligase RtcB homolog Q9Y3I0 Details Transitional endoplasmic reticulum ATPase P55072 Details Protein cereblon Q96SW2 Details Prohibitin-2 Q99623 Details Heterochromatin protein 1-binding protein 3 Q5SSJ5 Details Transgelin-3 Q9UI15 Details Eukaryotic translation initiation factor 2-alpha kinase 1 Q9BQI3 Details NAD-dependent protein deacetylase sirtuin-2 Q8IXJ6 Details V-type proton ATPase subunit D Q9Y5K8 Details V-type proton ATPase subunit H Q9UI12 Details